Driver Mutations Associated With Smoking And Other Environmental Factors: Prospective And Integrative Genomic Analysis From The Japan Molecularepidemiology For Lung Cancer Study (Jme).

7516 Background: Driver mutations are critical for lung cancer development as well as therapeutic targets. Its associations with environmental factors, sex hormones and virus remain elusive. Methods: This is a prospective, multicenter study on molecular epidemiology sponsored by the Japanese National Hospital Organization. Eligible are newly diagnosed patients with stage I to IIIB non-small cell lung cancer (NSCLC) who underwent surgery. Somatic mutations were examined by multiplex targeted deep sequencing (Illumina), validated by sensitive PCR methods, in surgical specimens, and human papilloma virus (HPV) using a PCR-based microarray system. The SWOG 0424 questionnaire was used to obtain information on patient demographics and detailed environmental factors. The sample size was 900 cases consisting of 450 ever-smokers and 450 never-smokers. Results: From July 2012 to December 2013, 958 patients were accrued from 43 institutions, and 901 samples were successfully tested for molecular analyses. Histology ...