Cell-type specific role of the RNA-binding protein, NONO, in the DNA double-strand break response in the mouse testes

•NONO is an RNA binding protein previously implicated in NHEJ repair.•DNA damage sensitivity was investigated in testes of NONO knockout (KO) mice.•In the absence of genotoxic stress, KO mice had at most a mild testes phenotype.•Sertoli cells of irradiated KO mice showed a number of markers of radiosensitivity.•Germ cells, which do not normally express NONO, died from loss of stromal support.