Rapid Flim: The New And Innovative Method For Ultra-Fast Imaging Of Biological Processes

Imaging the dynamics of life requires high spatial and temporal resolution. Therefore, expanding the combination of excellent time resolution from Fluorescence Lifetime Imaging (FLIM) based on Time-Correlated Single Photon Counting (TCSPC) with high spatial resolution towards ultra fast image acquisition times is most desirable. Speeding up FLIM enables to study processes and changes occurring on short time scales, such as signal transduction pathways in cells or fast moving sub-cellular structures.