Preliminary data from a longitudinal study of the airway microbiome in COPD
EUROPEAN RESPIRATORY JOURNAL(2015)
摘要
The longitudinal microbiome of COPD patients during periods of disease stability and during exacerbations remain to be studied on a large scale. This abstract reports preliminary data from a study of 173 COPD patients who were followed prospectively with sputum samples obtained at baseline, onset and end of exacerbations, and at 6 months follow-up.207 sputum samples were obtained (range 1-6 samples/patient). Sputum samples underwent 16S rRNA metagenomic sequencing on the Illumina MiSeq platform with identification of bacterial rRNA to species level.Preliminary data on the first 58 sputum samples from 45 patients have been analysed. Shannon Species Diversity indices ranged from 0.46 to 3.27, with the less diverse samples predominantly containing Haemophilus or Streptococcus species. Having 30% or greater Haemophilus reads was significantly associated with a higher GOLD stage (P=0.016). Principal coordinate analysis showed that there were distinct clusters of samples at the species level ![Figure][1] Regression analysis showed higher GOLD stage (A-D) was significantly associated with reduced species diversity (p=0.009). There was a trend to a relationship between FEV1 % predicted and species diversity.This work is the largest study characterising the COPD lung microbiome to date, providing longitudinal data during periods of stability and during exacerbations; demonstrating clinically important differences in microbiota between patients. [1]: pending:yes
更多查看译文
关键词
Bacteria,COPD - exacerbations,Infections
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络