Efficient production of an avian adeno-associated virus vector using insect cell/baculovirus expression system

•The AAAV proteins can be expressed efficiently in the rBac-infected insect cells.•The AAAV particles can be assembled correctly in insect cells without the helper viruses.•The AAAV vector produced in insect cells can mediate the expression of GFP in chicken cells.•The titer of rAAAV produced in insect cells is greatly improved.•A scalable and efficient platform for producing rAAAV has been established.