GW26-e1492 Changes of Small-conductance calcium-activated K(+) channels 3 (SK3) in Patients with Persistent Atrial Fibrillation

surgery without LAD ligation; b). ShamþH-PHC group: The rats were administrated with high dose of penehyclidine hydrochloride (H-PHC; 1 mg/kg bodyweight)by i.v. 30min before sham surgery; c). Ischemia reperfusion (I/R) group: The rats were subjected to a 30min LAD coronary artery ligation followed by 3h reperfusion; d). I/RþL-PHC group: The rats were administrated with low dose of PHC (L-PHC; 0.1 mg/kg bodyweight) 30min before I/R; e). I/RþMPHC group: The rats were administrated with moderate dose of PHC (M-PHC; 0.3 mg/kg bodyweight) 30min before I/R; f). I/RþH-PHC group: The rats were injected with H-PHC (1 mg/kg bodyweight) 30min before I/R. Cardiac function was measured by echocardiography after 3h reperfusion. Blood samples were collected. Then, the activities and levels of myocardial enzymes and antioxidant enzymes in serum were detected. Evans blue/TTC double staining was performed to assess infarct size. Cardiomyocyte apoptosis was evaluated by TUNEL assay. The release of inflammatory cytokines and inflammatory mediators was detected by ELISA. Western blot was performed to analyze the expression of COX-2, p-IkB, IkB and NF-kB.