Development of a real-time PCR for Bartonella spp. detection, a current emerging microorganism

Elena Parra,Ferran Segura, Jessica Tijero,Imma Pons,María-Mercedes Nogueras

Molecular and Cellular Probes（2017）

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摘要

A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100 ng–100 fg and sensitivity was 50 copies/reaction.

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关键词

Bartonella,Real-time PCR,Optimization,Zoonoses

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