Phylogenetic relationships of Thinopyrum and Triticum species revealed by SCoT and CDDP markers

To evaluate the phylogenetic relationships among Thinopyrum species and Triticum species, 7 accessions of Thinopyrum species (2 Th. bessarabicum , 1 Th. elongatum , 2 Th. intermedium and 2. Th. ponticum ), 11 accessions of Triticum species (2 Aeglips tauschii , 1 T. monococcum , 1 T. turgidum , 2 T. timopheevii and 5 T. aestivum ) together with Hordeum vulgare cv. Golden were analysed using 17 SCoT and 10 CDDP markers. The mean number of observed alleles was 8.5 and 6.6 among the species for SCoT and CDDP markers, respectively. Based on the genetic data produced by the SCoT and CDDP markers, cluster analysis among Thinopyrum species, Triticum species and Hordeum was performed to generate dendrograms. The genetic relationships among Thinopyrum species, Triticum species and H. vulgare revealed by the SCoT markers were in agreement with the results of the CDDP markers. On both dendrograms, these species formed three clusters. The results indicated that Thinopyrum species and Triticum species were the most closely related, whereas H. vulgare was relatively distant from both genera. In addition, seven markers, i.e. SCoT 9 , SCoT 31 , SCoT 34 , WRKY - R1 , WRKY - R2 , MADS - 1 and MADS - 4 were developed for monitoring introgression of Thinopyrum chromosomes or chromosome segments into Triticum species.