Flow-based sorting of neonatal lymphocyte populations for transcriptomics analysis

Ravi S. Misra,Soumyaroop Bhattacharya,Heidie L. Huyck,Jyh-Chiang E. Wang,Christopher G. Slaunwhite, Sharleen L. Slaunwhite, Terry R. Wightman,Shelley Secor-Socha,Sara K. Misra,Timothy P. Bushnell, Ann-Marie Reynolds,Rita M. Ryan,Sally A. Quataert,Gloria S. Pryhuber,Thomas J. Mariani

Journal of Immunological Methods（2016）

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摘要

Procedure diagram. Summary of the critical steps involved in the standard operating procedure (SOP) developed for this study. A detailed SOP is included in the Supplement.Image 1

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关键词

Flow sorting,BPD,Prematurity,Lymphocytes,T-Cell,PBMC,RNASeq

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