Antitumor Immune Response Passively Prevents And Eliminates Skin Tumors On The Mouse Model Of Human Papillomavirus Cancers

Infection by mouse papillomavirus (PV), MmuPV1, of T cell-deficient, B6.Cg-Foxn1nu/J nude mice revealed that four, distinct squamous papilloma phenotypes developed simultaneously after infection of experimental mice. Papillomas appeared on the muzzle, vagina, and tail at or about day 42 days post-inoculation. The dorsal skin developed papillomas and hair follicle tumors (trichoblastomas) as early as 26 days after infection. When this aggressive tumor was transplanted into normal congenic strains, the transplant immediately regressed while the transplanted tumor into B6.Cg-Foxn1nu/J continued to grow. This result suggested that T-cell deficiency is critical to tumor formation. Passive transfer of hyperimmune sera from normal congenic mice immunized with MmuPV1 virus-like particles (VLPs) to T cell-deficient strains of mice prevented infection by virions of experimental mice. A cell adoption study showed that activated CD8+ populations collected during the regression process of MmuPV1 tumor explants efficiently eliminated both growing and established tumors in the model. Consequently, this study will provide an efficient immunotherapeutic strategy that can be employed to HPV-positive patients, who are at a risk of cancer development. Citation Format: Joongho Joh, Shinje Ghim, Paula M. Chilton, Jino Park, Sarah A. Wilcher, Mary L. Proctor, Alfred B. Jenson. Antitumor immune response passively prevents and eliminates skin tumors on the mouse model of human papillomavirus cancers. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr LB-297.