Differential Screening Using High Density Filters Reveals A Dramatic Change In Gene Expression At Different Growth Phase In Arabidopsis Cell Suspension Cultures

Differential screening using high density filters prepared from ordered cDNA libraries has been tested in order to obtain an overall view of plant genes expressed in different tissues or under particular physiological conditions. Arabidopsis thaliana cell suspension cultures at different growth phases were used for this approach. Our results indicate that there are dramatic changes in gene expression during the different growth phases of these cell suspension cultures. The analysis by partial sequencing of a sample of 61 cDNA clones specifically or preferentially expressed in the stationary phase, reveals that most of these clones correspond to genes having a putative relationship with mechanisms involved in response to stress, in defence against pathogens or in senescence processes.