Effect of High Pressure-Enzymolysis on Solubility and Structural Characteristic of Rice Protein

Thermo denatured rice protein was treated by 100 MPa for 10 min at 25℃ and then was hydrolyzed with Alcalase.The solubility and structural characterization of the protein and its hydrolysates were analyzed by size exclusion-high performance liquid chromatography(SE-HPLC),Fourier transform infrared spectroscopy(FTIR)and scanning electric microscope(SEM)so as to evaluate the effect of high pressure.The results showed that the solubility of hydrolyzed rice protein after high pressure treated could elevate to75.33%from 58.9% of non-high pressure treated.The 57-105 ku fraction of the protein showed a dissolved state after high pressure treatment,and the contents of 4.4and 2.0ku fractions increased during the hydrolysis of the protein,which were different from that of non-high pressure treated.Theβ-sheet andβ-turn were the major conformation of the protein.The structure of the protein body particles became loosen and easier to be hydrolyzed after high pressure treatment.These results show that 100 MPa treatment could promote the enzymatic hydrolysis of thermo-denatured rice protein.