Changes of proliferation and phenotypes of epithelial cells and fibroblasts co-cultured on SF/PLLA composite fibrous scaffolds

Objective To observe the influence of SF/PLLA composite fibrous scaffolds with different quality ratios of SF and PLLA in the proliferation and phenotypes of epithelial cells and fibroblasts,and to clarify the cytocompatibility of SF/PLLA bioengineering membrane.Methods The SF and PLLA were served as materials to construct composite scaffolds.The different proportions of SF/PLLA nanofibers membrane were compounded with different ratios of SF and PLLA(S50/P50,S40/P60 and S30/P70)and mixed spinning.The primary epithelial cells and fibroblasts from rat skin tissue were cultured on the SF/PLLA composite scaffolds.CCK8 test was used to evaluate the cell proliferation,and immunofluorescence staining was used to determine the expressions of the specific marker proteins of cultured cells.Results The proliferation activities of fibroblasts in S50/P50 group were significantly higher than those in S40/P60 and S30/P70 groups,and the proliferation activities of epithelial cells on the composite scaffolds in S40/P60 group were higher than those in S50/P50 and S30/P70groups(P0.01)on the5 th and 6th days.The epithelial cells cultured on the SF/PLLA composite scaffolds expressed CK 19 protein and the fibroblasts expressed Vimentin protein. When two kinds of cells were co-cultured on SF/PLLA composite fibrous scaffolds,the cell growth status in S40/P60 and S50/P50 groups were superior to that in S30/P70 group.Conclusion The increasing of SF concentration in composite scaffolds can obviously increase the cell proliferation activity and promote the cell survival;SF/PLLA scaffolds has no significant influence in the cell phenotype.