Bioinformatics Analysis of WRKY31 Homologs in Soybean Genome

The gene family of WRKY is transcription factor that can be induced and involved in the response to biotic and abiotic stresses,and is conserved evolutionarily. In this research,the full-length cDNA of GmWRKY31 was cloned and bioinformatics analysis was used to predict the potential function of GmWRKY31. Phylogenetic analysis of GmWRKY31 and its homologs with different plant species were conducted by the software MEGA 5. 0. The results showed that three copies of WRKY31 exited in the soybean genome,located on the chromosome 6,12 and 13. GmWRKY31 belongs to the type II WRKY transcription factors,with a WRKY domain and a C2H2-type zinc finger. Real-time RT-PCR analysis showed that GmWRKY31 was expressed in soybean leaf,flower,pod,root,nodule,and the flower had the highest expression,the lowest expression was in the pod. The result of the phylogenetic tree indicted that WRKY31 from Glycine max and Phaseolus vulgaris were the nearest in genetic relationship. While Glyma06g46420. 2 and Glyma12g10350. 1(the other two copies of WRKY31 of Glycine max) had closer relationship. The result of protein structure and function prediction showed that GmWRKY31 was a transcription factor being involved in the stress response of plant,however,the phylogenetic tree indicted that significant difference of the WRKY genes happened in evolution of soybean.