Value of dynamic monitoring of HLA I and procalcitonin on differentiation of acute rejection from infection after renal transplantation

Objective To investigate the value of human leukocyte antigen(HLA)Ⅰon peripheral lymphocytes and procalcitonin(PCT)level on differential diagnosis of acute rejection(AR)and infection after renal transplantation.Methods Ninety-nine renal transplant recipients(102 cases)were divided into 3 groups,according to clinical symptoms,renal function examination,imaging and renal allograft biopsy after transplantation.There were 18 cases in AR group,14 cases in infection group and 70 cases in post-transplantation normal group.Twenty healthy blood donors in Qilu hospital were selected as control group.The level of HLA Ⅰ on peripheral lymphocytes was detected by flow cytometry(FCM)and the level of PCT in serum was detected by immunofluorescence.Results The level of HLA Ⅰin patients who underwent AR or severe bacterial infection after renal transplantation was increased,but there was no significant difference between the two groups(P0.05).The positive rate of PCT in infection group was significantly higher than that in AR group(P0.01).Conclusions Compared with the level of HLA Ⅰon peripheral lymphocytes,the changes of plasma PCT level was more sensitive to differentiate AR from severe bacterial infection after renal transplantation.