AB040. Early and long-term protective effect of uncultured adipose stromal vascular fraction against renal ischemia-reperfusion injury via pre-ischemic administration

BackgroundIschemia-reperfusion (IR) induced acute kidney injury (AKI) is the common clinical syndrome. Stem/progenitor cells therapy is a promising option to foster the intrinsic capacity of renal regeneration. However, several challenges still remain due to the potential risks during in vitro cell culture, low retention rate after transplantation and unclear effect on the progression of chronic kidney disease (CKD). Adipose stromal vascular fraction (SVF) is regarded as an attractive cell source for cell-based therapy without need of in vitro expansion. Preconditioning with ischemia has been suggested to be a useful method to induce a delay of lethal cell injury, enhance cellular tolerance to injurious effects of IR, and promote cell retention and survival. The purpose of this study is to investigate the effect of pre-administration of uncultured adipose SVF on IR induced AKI and progression of fibrosis.