Quantifying Current Events Identifies a Novel Endurance Regulator
Trends in Microbiology(2016)
摘要
In nongrowing microbes, proteome turnover is reduced and identification of newly synthesized, low-abundance proteins is challenging. Babin and colleagues recently utilized bio-orthogonal noncanonical amino acid tagging (BONCAT) to identify actively synthesized proteins in nongrowing Pseudomonas aeruginosa, discovering a regulator whose influences range from biofilm formation to secondary metabolism.
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关键词
Pseudomonas aeruginosa,BONCAT,transcription,SutA,nongrowing cells
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