Male infertility in mice lacking the store-operated Ca2+ channel Orai1

•Orai1 knockout male mice are sterile.•Mouse testicular cells, including germ cells, have robust store-operated calcium entry, and this is completely lost in Orai1 knockout mice.•In the testes of Orai1 knockout mice, mature sperm fail to develop fully or survive.•Orai1-mediated Ca2+ entry appears necessary for the late stages of sperm development.