In Vitro Confocal Raman Micro-Spectroscopic Approach To Screen The Mode Of Action Of Chinese Herbs On Oxidative Damaged Human Sperm

This paper presented a rapid and reliable approach to screen the antioxidant active ingredients on human sperm from Chinese herbs by means of confocal Raman micro-spectroscopy. For the first time with the proposed approach, we performed the studies of rationalizing the active ingredients from several Chinese herbs. In this study, the FeSO4/H2O2-induced oxidative damaged sperm after co-culture with compounds or active fraction extracted from some Chinese herbs commonly used in the treatment of male infertility were observed by using confocal Raman micro-spectroscopy. The Raman spectral fingerprints of the damaged sperm were very different from the normal ones, and the further statistical analysis were successfully used to support the results, these differences were shorten after co-culture with the compounds or active fraction we had chosen, and the results were almost consistent with the traditional clinical trial results and other published data, all which showed that these compounds or active fraction were the chemogenomics of the Chinese herbs on anti-oxidative damaged sperm. In brief, we demonstrated that by means of confocal Raman micro-spectroscopy, in vitro is a rapid, reliable and large-scale approach to screen the antioxidant active ingredients on human sperm from Chinese herbs. Copyright (c) 2016 John Wiley & Sons, Ltd.