Corticosteroid-binding globulin (CBG) reactive centre loop antibodies and surface plasmon resonance interrogate the proposed heat dependent "flip-flop" mechanism of human CBG.

John G. Lewis,Rayleen Fredericks,Conan J. Fee,Peter A. Elder

The Journal of Steroid Biochemistry and Molecular Biology（2016）

引用 10|浏览6

暂无评分

摘要

•We interrogate the proposed heat dependent “flip-flop” of the reactive centre loop (RCL) of human CBG using antibodies and surface plasmon resonance.•These antibodies span 11 of the 18 residues of the RCL.•Surface plasmon resonance shows that up to 40°C the RCL remains accessible to RCL antibodies.•The release of cortisol from CBG by heat may be independent of the RCL

查看译文

关键词

Corticosteroid-binding globulin,CBG,Serpins,Elastase,Reactive centre loop,Cortisol,Temperature

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要