IN VITRO AND IN VIVO EVALUATION OF THE ANTIOXIDANT, ANTIDIABETIC AND CARDIOPROTECTIVE ACTIVITIES FROM THE AERIAL PARTS OF Anvillea radiata Coss. & Dur.

Since antidiabetic drugs currently used for glycemic control in diabetic patients cannot generally prevent long-term complications, new adjunctive, plant-derived therapies are under intensive research. This study investigated the properties of Anvillea radiata Coss. u0026 Dur (1) used in Algerian Sahara as a new source of antioxidant and antidiabetic compounds. Extracts of A. radiata. were prepared in several solvents and the amounts of phenolic compounds were quantified by HPLC and compared to standard phenolic antioxidants. The properties of these extracts were first screened in a wide array of tests, including standard antioxidant assays, inhibition of key digestive enzymes, cytotoxicity on A549 human adenocarcinomic epithelial cells, and superoxide and hydroxyl radical scavenging using spin trapping/EPR. The results showed n-butanolic and water A. radiata extracts to be very efficient antioxidants and promising antidiabetic compounds with α-amylase and α-glucosidase inhibitory effects, and to exert protective properties on the viability and insulin secretory function of pancreatic INS-1E beta cells. The positive effects of A. radiata extracts against hyperglycemia, hyperinsulinemia, and systemic and tissue response to oxidative stress (ie, protein carbonyls formation, lipid peroxidation and antioxidant depletion) induced by oral treatment of diabetic C57BL/6J mice fed with a high-fat and high-sucrose diet will be presented