Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics.

Roslyn M Bill,Annegret Ulke-Lemée,Stephanie P Cartwright,Rena Far, Jay Kim,Justin A MacDonald

Methods in molecular biology (Clifton, N.J.)（2019）

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摘要

New mass spectrometry approaches enable antibody-independent tracking of protein production. Herein, we outline an antibody-independent mass spectrometry method for tracking recombinant protein production in the methylotrophic yeast Pichia pastoris system.

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关键词

DAPK3,MRM-MS,Mass spectrometry,Multiple reaction monitoring,Recombinant protein production,Yeast,ZIPK

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