An improved and easy protocol for primary epithelial cell culture from atretic tissue in biliary atresia.

•Primary culture of extrahepatic biliary epithelial cell is difficult to achieve.•The new culture protocol produced 100% success rate.•No previous studies validated their culture protocol in so many patients.•Culture may solve the problem of tissue/cell availability for research in biliary atresia.•Cultured cells may help to design tissue engineering based grafts for management of above condition.