Effects Of Tracheal Occlusion On The Neonatal Cardiopulmonary Transition In An Ovine Model Of Diaphragmatic Hernia

Objective Fetoscopic endoluminal tracheal occlusion (FETO) aims to reverse pulmonary hypoplasia associated with congenital diaphragmatic hernia (CDH) and mitigate the associated respiratory insufficiency and pulmonary hypertension after birth. We aimed to determine whether FETO improves the cardiopulmonary transition at birth in an ovine model of CDH.Methods In 12 ovine fetuses with surgically induced diaphragmatic hernia (DH; 80 dGA), an endotracheal balloon was placed tracheoscopically at similar to 110 dGA and removed at approximate to 131 dGA (DH+FETO), while 10 were left untreated (DH). At approximate to 138 dGA, all lambs (survival at delivery: 67% [DH+FETO], 70% [DH]) were delivered via caesarean section and ventilated for 2 hours. Physiological and ventilation parameters were continuously recorded, and arterial blood-gas values were measured.Results Compared with DH, DH+FETO lambs had increased wet lung-to-body-weight ratio (0.031 +/- 0.004 vs 0.016 +/- 0.002) and dynamic lung compliance (0.7 +/- 0.1 vs 0.4 +/- 0.1 mL/cmH(2)O). Pulmonary vascular resistance was lower in DH+FETO lambs (0.44 +/- 0.11 vs 1.06 +/- 0.17 mm Hg/[mL/min]). However, after correction for lung weight, pulmonary blood flow was not significantly different between the groups (4.19 +/- 0.57 vs 4.05 +/- 0.60 mL/min/g). Alveolar-arterial difference in oxygen tension was not significantly different between DH+FETO and DH (402 +/- 41mm Hg vs 401 +/- 45 mm Hg).Conclusions FETO accelerated lung growth in fetuses with CDH and improved neonatal respiratory function during the cardiopulmonary transition at birth. However, despite improved lung compliance and reduced pulmonary vascular resistance, there were less pronounced benefits for gas exchange during the first 2 hours of life.