Validation of a site-specific recombination cloning technique for the rapid development of a full-length cDNA clone of a virulent field strain of vesicular stomatitis New Jersey virus.

Lauro Velazquez-Salinas,Steven J Pauszek,Jose Barrera,Benjamin A Clark,Manuel V Borca,Antonio Verdugo-Rodriguez,Carolina Stenfeldt,Jonathan Arzt,Luis L Rodriguez

Journal of Virological Methods（2019）

引用 5|浏览69

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摘要

•A full-length clone system to produce infectious VSV was constructed by site-specific recombination.•The virulence of rescued VSV was evaluated in pigs.•Site-specific recombination represents a useful and rapid methodology for cloning vesiculoviruses.

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关键词

Vesicular stomatitis virus,Site-specific recombination,In-fusion,cDNA clone

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