Universal retention standard for peptide separations using various modes of high-performance liquid chromatography.

Nicole Klaassen,Victor Spicer,Oleg V Krokhin

Journal of Chromatography A（2019）

引用 14|浏览13

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摘要

•Modeling peptide properties helps the development of peptide retention standard.•The standard can be used for the most popular peptide separations modes.•The list includes: RPHPLC, HILIC, strong anion and cation exchange, CZE.•Peptides have nearly identical mass and intense y ion to simplify MS assignments.

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关键词

Peptide HPLC,Hydrophilic interaction liquid chromatography,Strong cation exchange,Strong anion exchange,Reversed-phase chromatography,Peptide retention time prediction,Retention standards

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