Increased IL-17 and/or IFN-γ producing T cell subsets in gut mucosa of long-term treated HIV-1-infected women.

Objective: The influence of sex on gut mucosal T-cell response in HIV-1 infection remains largely unknown. We explored whether the frequencies of interferon-gamma and/or IL-17 producing naive, T central memory and T effector memory (TEM) CD4(+) (Th1, Th17) and CD8(+) T (Tc1, Tc17) cells measured in gut and peripheral districts differed between female and male HIV-1-infected patients. Methods: Thirty long-term-treated HIV-1-infected individuals were enrolled. The frequencies of Th1, Th17, Tc1, Tc17-cell subsets (single and double) were evaluated by multiparametric flow cytometry in lamina propria lymphocytes and peripheral blood mononuclear cells (PBMC). Results: A sex-based pattern was recorded in the differences of Th1, Th17, Tc1, Tc17-cell subset (single and double) frequencies between gut and peripheral blood. Female patients had stronger alterations in the gut mucosal T-cell repertoire, especially increased Th1, Th17, and Th1/Th17-cell subset frequencies, compared with the blood district than their male counterparts. Higher naive Tc1, Tc17, Tc1/Tc17, TEM Tc17, and TEM Tc1/Tc17 levels were also recorded in the gut mucosa than in the PBMC of HIV-1-infected women. Males and females also differed in their gut T-cell response, with women being characterized by higher Th1, Th17, Tc1, Tc17, and Th1/Th17 cells subset levels than men. By contrast, only TEM Th1/Th17 and TEM Tc17 in PBMC differed between males and females. Conclusion: Sex-based differences observed in the gut T-cell response of HIV-1-infected patients might contribute to the disease dimorphism. Copyright (C) 2019 Wolters Kluwer Health, Inc. All rights reserved.