Remarkable complexity and variability of corticospinal tract defects in adult Semaphorin 6A knockout mice.

•Adult Sema6A knockout mice have corticospinal tract (CST) defects.•The aberrant trajectories were evaluated by 3D reconstruction of PKCγ-stained fibers.•The CST defects in Sema6A knockout mice were highly complex and variable.•3D reconstruction is a powerful method to visualize these CST axon guidance defects.