Full-Length Open Reading Frame Amplification of Hepatitis C Virus

    Ulrik Fahnøe
    Ulrik Fahnøe

    Methods in molecular biology (Clifton, N.J.), Volume 1911, 2019, Pages 85-91.

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    Keywords:
    CloningFull-length ORF RT-PCRHCVHepatitis C virusLong PCRMore(3+)

    Abstract:

    The purpose of this method is to amplify the full coding sequence of hepatitis C virus (HCV) by a single round reverse transcriptase-polymerase chain reaction (RT-PCR) approach. Our method relies on a highly robust and sensitive RNA extraction procedure and cutting-edge RT-PCR enzymes, all of which have been rigorously tested and optimize...More

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