Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform.

•Ammonia measurement on Roche Cobas 8000 suffers from 11,3% sample absorbance error flags for exceeding the absorbance limits on the detector.•AMC NH3L adaptation solves 78% of absorbance errors of Roche method and forhigh ammonia concentration, is more robust to endogenous inferences.•AMC NH3L adaptation is a viable alternative to the Roche protocol for the ammonia measurement.