Effects of pilose antler peptide on bleomycin-induced pulmonary fibrosis in mice.

The aim of present study was to evaluate the effects of the pilose antler peptide (PAP) on bleomycin (BLM)-induced lung fibrosis. The lung wet-to-dry weight (W/D) ratio and myeloperoxidase (MPO) activity were measured. The serum levels of super-oxide dismutase (SOD), malondialdehyde (MDA) were examined. Then the contents of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and IL-6 were determined using ELISA method. Furthermore, the protein expressions of Rho, ROCK1, p-I kappa B, I kappa B, p-NF-kappa B, NF-kappa B in lung tissues were detected by western blot analysis. As a result, PAP markedly decreased pulmonary W/D ratio, lung MPO activity and relieved lung histopathological changes. In addition, PAP increased the level of SOD and reduced the levels of MDA, TNF-alpha, IL-1 beta, IL-6 in serum of BLM-stimulated mice. In addition, PAP remarkably inhibited the protein levels of ROCK/NF-kappa B pathway. In conclusion, our results showed that PAP exhibited protective effects on pulmonary fibrosis via the regulation of ROCK/NF-kappa B pathway.