Ideal versus real automated twin column recycling chromatography process.
Journal of Chromatography A（2017）
摘要
•A twin-column recycling separation process (TCRSP) device was built for LC separation.•It is connected and automated by a UHPLC instrument to resolve tough separations.•The actual performance of the TCRSP deviates from that of the ideal TCRSP.•This deviation is due to the pressure-dependent retention of the analytes.•The change of molar volume of benzo[a]anthracene/chrysene isomers is −10cm3/mol.
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关键词
Recycling chromatography,Ideal versus real recycling process,High-resolution liquid chromatography,Reversed-phase liquid chromatography,Planar polycyclic aromatic hydrocarbon isomers,Pressure-dependent retention factor,Partial molar volume
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