Controlling Capillary-Driven Fluid Transport in Paper-Based Microfluidic Devices Using a Movable Valve.

This paper describes a novel, strategy for fabricating the movable valve on paper-based microfluidic devices to manipulate capillary-driven fluids. The movable valve fabrication is first realized using hollow rivets as the-holding center it, control the paper channel in different layer movement that Jesuits in the :channel's connection or disconnection. The relatively simple Valve fabrication procedure is robust, Versatile, and, compatible with microfluidic paper-based analytical devices (mu PADs) with differing levels of complexity. It is remarkable that the movable valve can be convenient and free to control fluid without the timing setting; advantages that make it user-friendly for untrained users to carry out the complex multistep operations. For the, performance of the Movable valve to be-verified, several different designs of mu PADs were tested and obtained with satisfactory results. In addition; in the proof-of-concept enzyme-linked immunosorbent assay experiments, we demonstrate the use of these valves in mu PADs for the successful analysis of samples of carcino-embryonic antigen, showing good sensitivity and reproducibility. We hope this technique will open new avenues for the fabrication of paper-based valves in an easily adoptable and widely available way on mu PADs and provide potential point-of-Care applications in the future.