Biochemical and milk-clotting properties and mapping of catalytic subsites of an extracellular aspartic peptidase from basidiomycete fungus Phanerochaete chrysosporium.

•Absence of proteolysis at alkaline pH.•Aspartic peptidase and its potential to milk clotting.•Mapping of catalytic subsites and on usefulness for cheese production.•Phanerochaete chrysosporium-secreted acidic peptidase.