Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies

Michele Chirichella,Simonetta Lisi,Marco Fantini,Martina Goracci,Mariantonietta Calvello,Rossella Brandi,Ivan Arisi,Mara D'Onofrio,Cristina Di Primio,Antonino Cattaneo

NATURE METHODS（2017）

引用 9|浏览24

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摘要

PISA, generates intrabodies that selectively bind and interfere with the intracellular function of an acetylated version of a target protein.

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关键词

Acetylation,Antibody generation,Antibody isolation and purification,High-throughput screening,Life Sciences,general,Biological Techniques,Biological Microscopy,Biomedical Engineering/Biotechnology,Bioinformatics,Proteomics

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