First detection of Leishmania kDNA in canine cerumen samples by qPCR.

Silvia Belinchón-Lorenzo,Juan Carlos Parejo,Virginia Iniesta,Javier Fernández-Cotrina,Rubén Muñoz-Madrid,Isabel Monroy,Victoria Baz,Adela Gómez-Luque,Francisco Javier Serrano-Aguilera,José Luis Barneto,Luis Carlos Gómez-Nieto

Veterinary Parasitology（2016）

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摘要

•Leishmania kDNA was detected for the first time in cerumen of dogs with leishmaniasis.•Sensitivity of cerumen was 90.9%, higher than blood and bone marrow.•The collection of cerumen is harmless, fast and easy.•Cerumen does not need specific conditions for transport and storage.•Cerumen-qPCR method has a great potential in the diagnostic of canine leishmaniosis.

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关键词

Cerumen,Leishmania infantum,Dog,Kinetoplast DNA (kDNA),Real Time PCR

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