Streptomyces Camponoticapitis Sp Nov., An Actinomycete Isolated From The Head Of An Ant (Camponotus Japonicus Mayr)

A novel single-spore-producing actinomycete, designated strain 2H-TWYE14(T), was isolated from the head of an ant (Camponotus japonicus Mayr) and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain 2H-TWYE14(T) belongs to the genus Streptomyces, with highest sequence similarity to Streptomyces niveus NRRL 2466(T) (98.84 %). Analysis based on the gyrB gene also indicated that strain 2H-TWYE14(T) should be assigned to the genus Streptomyces. The chemotaxonomic properties of strain 2H-TWYE14(T) were consistent with those of members of the genus Streptomyces. The cell wall contained LL-diaminopimelic acid. The predominant menaquinones were MK-9(H-6), MK-9(H-8) and MK-9 (H-4). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were iso-C-16:0 and iso-C-15:0. DNA-DNA hybridization experiments and phenotypic tests were carried out between strain 2H-TWYE14(T) and its phylogenetically closely related strain S. niveus JCM 4251(T), which further clarified their relatedness and demonstrated that 2H-TWYE14(T) could be distinguished from S. niveus. Therefore, it is concluded that strain 2H-TWYE14(T) can be classified as representing a novel species of the genus Streptomyces, for which the name Streptomyces camponoticapitis sp. nov. is proposed. The type strain is 2H-TWYE14(T) (=DSM 100523(T) =CGMCC 4.7275(T)).