Impact of preparation method and variables on the internal structure, morphology, and presence of liposomes in phytantriol-Pluronic(®) F127 cubosomes.

•Comparison of phytantriol cubosomes prepared by top-down and bottom-up approaches.•Cubosomes with 20% F127 had optimized enhanced colloidal stability over 4 months.•Cryo-TEM images of top-down cubosomes displayed fewer liposomes.•Robust approach to yield primarily cubosomes in dispersions.