Complete NMR assignment of a bisecting hybrid-type oligosaccharide transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase.

Takashi Yamanoi,Yoshiki Oda,Kaname Katsuraya,Toshiyuki Inazu,Kenji Yamamoto

Carbohydrate Research（2016）

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摘要

•The nuclear magnetic resonance spectral assignment of a bisecting hybrid-type oligosaccharide, transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase (Endo-M).•The complete NMR assignment of a bisecting hybrid-type oligosaccharide through 1H- and 13C-NMR, DQF-COSY, HSQC, HMBC, TOCSY, and NOESY experiments.•The determination of the structure of the glycoside linkage formed by the Endo-M transglycosylation.

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关键词

NMR spectral assignment,Bisecting hybrid-type oligosaccharide,Endo-β-N-acetylglucosaminidase,Endo-M,Transglycosylation product

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