Genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria arborescens
Archives of virology(2016)
摘要
The phytopathogenic fungus Alternaria spp. contains a variety of double-stranded RNA (dsRNA) elements of different sizes. Detailed analysis of next-generation sequencing data obtained using dsRNA purified from Alternaria arborescens , from which we had previously found Alternaria arborescens victorivirus 1, revealed the presence of another mycoviral-like dsRNA of approximately 2.5 kbp in length. When using the fungal mitochondrial genetic code, this dsRNA has a single open reading frame that potentially encodes an RNA-dependent RNA polymerase (RdRp) with significant to sequence similarity to those of viruses of the genus Mitovirus . Moreover, both the 5’- and 3’-untranslated regions have the potential to fold into stable stem-loop structures, which is characteristic of mitoviruses. Pairwise comparisons and phylogenetic analysis of the deduced amino acid sequences of RdRp indicated that the virus we identified in A. arborescens is a distinct member of the genus Mitovirus in the family Narnaviridae , designated as “Alternaria arborescens mitovirus 1” (AaMV1).
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关键词
Single Open Reading Frame, Host Fungus, Tomato Pathotype, Japanese Pear Pathotypes, Alternaria Arborescens
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