Anthocyanin Contents In The Seed Coat Of Black Soya Bean And Their Anti-Human Tyrosinase Activity And Antioxidative Activity

ObjectiveThe seed coat of black soya bean (SCBS) contains high amount of anthocyanins and shows antioxidant and anti-mushroom tyrosinase activities. The objectives of this study were to analyse the anthocyanins in SCBS with different solvents and to find the relationship between anthocyanin profile with anti-human and anti-mushroom tyrosinase activities.MethodsSCBS was extracted with hot water, 50 and 80% ethanol, 50 and 80% acetone and 50 and 80% acidified acetone. Total phenol and total flavonoid contents in the extracts were determined. Anthocyanins in the extracts were analysed using HPLC and LC/MS/MS. A genetically engineered human tyrosinase was used to evaluate the anti-tyrosinase potential of the extracts from SCBS.Results80% acetone extract from SCBS obtained the highest total phenol, total flavonoid and cyanidin-3-O-glucoside (C3G) contents among all the extracts, whereas the hot water extract showed the lowest antioxidant contents. Three anthocyanin compounds were found in all the extracts from SCBS, and the analysis of HPLC and LC/MS/MS indicated that they were C3G, delphinidin-3-O-glucoside (D3G) and peonidin-3-O-glucoside (P3G). The ratios of C3G (2.84 mg g(-1)), D3G (0.34 mg g(-1)) and P3G (0.35 mg g(-1)) in 80% acidified acetone extract were 76.6, 9.1 and 9.3%, respectively. All the extracts from SCBS possessed anti-human tyrosinase activity. Moreover, a good correlation was found between the anti-human tyrosinase activities and C3G contents in the extracts.ConclusionAntioxidants in SCBS also possess anti-human and anti-mushroom tyrosinase activities.ResumeObjectifL'enveloppe de la graine du soja noir (SCBS) contient une quantite elevee des anthocyanes et possedent des activites antioxydantes et anti-tyrosinase de champignon. Les objectifs de cette etude etaient d'analyser les anthocyanes dans SCBS avec differents solvants et de trouver la relation entre le profil des anthocyanes avec les activites anti-tyrosinase humaine et champignon.MethodesSCBS a ete extrait a l'eau chaude, a l'ethanol a 50 et 80%, a l'acetone 50 et 80% et a l' d'acetone acidifiee a 50 and 80%. Les phenols et le total des flavonoides contenus dans les extraits ont ete determines. Les Anthocyanins dans les extraits ont ete analysees par HPLC et LC / MS / MS. La tyrosinase humaine obtenue par genie genetique a ete utilisee pour evaluer le potentiel anti-tyrosinase des extraits de SCBS.ResultatsL'extrait a l'acetone 80% a partir du SCBS a montre la plus haute teneur en phenols, en flavonoides, et en cyanidine-3-O-glucoside (C3G) entre tous les extraits, alors que l'extrait a l'eau chaude a montre les plus basses concentrations en antioxydants. Trois composes anthocyanes ont ete trouves dans tous les extraits de SCBS, et l'analyse de HPLC et LC / MS / MS a indique qu'ils representent C3G, delphinidine-3-O-glucoside (D3G) et peonidine-3-O-glucoside (P3G). Les rapports des C3G (2,84 mg / g), D3G (0,34 mg / g) et de P3G (0,35 mg / g) dans l'extrait 80% d'acetone acidifie etaient de 76,6, 9,1 et 9,3%, respectivement. Tous les extraits de SCBS possedaient une activite anti-tyrosinase humaine. En outre, une bonne correlation a ete trouvee entre les activites anti-tyrosinase humaine et les contenus C3G dans les extraits.ConclusionLes antioxydants dans SCBS possedent des deux activites anti-tyrosinase humaine et champignon.