Long Noncoding RNA Plasmacytoma Variant Translocation 1 (PVT1) Promotes Colon Cancer Progression via Endogenous Sponging miR-26b.

Background: Recently, long noncoding RNAs (IncRNAs) have received wide attention in the area of tumor progression. Dysregulation of IncRNAs has been shown to participated in colon cancer, a known malignant tumor. This study aimed to identify the way IncRNA PVT1 affects the progression of colon cancer. Material/Methods: Both human colon cancer tissues and 30 paired adjacent normal tissue samples, as well as the colon cancer cells, were collected. Then quantitative real-time (qRT-PCR) was performed to detect the expression of IncRNA PVT1 and miR-26b. Furthermore, the role of PVT1 was determined by function assays such as cell proliferation assay, invasion assay, and wound healing assay. The mechanism was studied using western blot assay and luciferase assay. Results: We demonstrate that the expression of PVT1 was significantly higher in tumor tissue compared with the ad- jacent normal tissue with a lower expression of miR-26b. Moreover, PVT1 promoted tumor growth, migration, and invasion in vitro. In addition, further experiments revealed that miR-26b was a direct target of PVT1 and could inhibit cell migration, invasion, and proliferation in colon cancer. Conclusions: Our results suggest that PVT1 could promote metastasis and proliferation of colon cancer via endogenous sponging and inhibiting the expression of miR-26b, which may highlight the significance of IncRNA PVT1 in colon cancer tumorigenesis.