Cell-type specific polysome profiling from mammalian tissues.

Joseph Seimetz,Waqar Arif,Sushant Bangru,Mikel Hernaez,Auinash Kalsotra

Methods（2019）

引用 14|浏览5

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摘要

•Comparison of different methods to survey translation at a genome-wide scale.•Isolation of tissues and purified cell-types for polysome profiling.•A detailed protocol of polysome fractionation for RNA-sequencing.•Bioinformatics workflow (Shirloc) for determining differential ribosome occupancy of transcripts.

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关键词

Cell-type isolation from tissues,Polysome profiling,Next-generation sequencing and bioinformatics,Ribosomal occupancy shift,Translation regulation

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