Zinc clasp-based reversible toolset for selective metal-mediated protein heterodimerization.
CHEMICAL COMMUNICATIONS(2018)
摘要
Considering the complex biological quandaries of the tightly woven networks of biological macromolecules, we present an optimized zinc clasp-based toolset from the CD4 co-receptor and Lck protein tyrosine kinase complex for selective, tight and fully reversible protein heterodimerization (logK(12) = 18.6). We demonstrated its utility on CD4-tagged proteins with capture from bacterial lysate and constructed molecular baits using a new small-molecule tether.
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关键词
protein heterodimerization,zinc,reversible toolset,clasp-based,metal-mediated
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