Protocol for Agrobacterium-Mediated Transformation and Transgenic Plant Production of Switchgrass.

Switchgrass (Panicum virgatum L.) is one of the most important bioenergy crops for lignocellulose ethanol production. Molecular breeding provides a powerful tool to supplement conventional switchgrass breeding by introducing or editing genes of interest. In this chapter, we describe Agrobacterium tumefaciens-mediated transformation protocols for lowland tetraploid switchgrass cultivar Alamo.