A facile method to oriented immobilization of His-tagged BirA on Co 3+ -NTA agarose beads.

Li Xu, Rou Wang,Hanyu Cao,Ting Xu,Lulu Han,Chundong Huang,Lingyun Jia

Enzyme and Microbial Technology（2019）

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摘要

•A facile and economical method was established for oriented immobilization of BirA.•BirA was oriented immobilized through H2O2 oxidation of Co2+ and His-tag.•His-tag of BirA at N-terminal exposed better immobilization than that at C-terminal.•The immobilized BirA exhibited the same activity as the free.•The immobilized His-BirA showed less pH-sensitive and preferable thermo-stability.

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关键词

Immobilization,Biotin ligase,Co3+,H2O2

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