Pancreatic Microtumors: A Novel 3D Ex Vivo Testing Platform.
Methods in molecular biology (Clifton, N.J.)(2019)
摘要
The tumor microenvironment plays a vital role in therapeutic resistance in pancreatic adenocarcinoma. We have created a 3D model to study the tumor microenvironment ex vivo that integrates tumor cells with tumor associated macrophages (TAMs) and cancer associated fibroblasts (CAFs). The microtumor model recreates the complex histology of a primary tumor and can be analyzed histologically or with viability assays for therapeutic testing. Microtumors demonstrate increased resistance to gemcitabine when compared to tumor cells alone in a 3D spheroid, highlighting the contribution of TAMs and CAFs when designing models for therapeutic testing.
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关键词
3D culture,Cancer associated fibroblast (CAF),Microenvironment,Spheroid,Tumor associated macrophage (TAM)
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