Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography – electrospray ionization – mass spectrometry

Karina M Tonon,Antonio Miranda,Ana Cristina F V Abrão,Mauro B de Morais,Tania B Morais

Food Chemistry（2019）

引用 54|浏览14

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摘要

•A method for the analysis of typical HMOs was validated.•Neutral and acidic HMOs are concomitantly quantified in the same analysis.•The method was applied to analyze HMOs in real human milk samples.•Accurate and reliable quantification of HMOs was achieved.•The method allows the Lewis and Secretor phenotype of the mother to be determined.

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关键词

2′-Fucosyllactose (PubChem CID: 170484),3′-Fucosyllactose (PubChem CID: 161460),Lacto-N-fucopentaose I (PubChem CID: 50909802),Lacto-N-difucohexaose I (PubChem CID: 3082109),Lacto-N-difucohexaose II (PubChem CID: 53262301),Lacto-N-tetraose (PubChem CID: 440993),Lacto-N-hexaose (PubChem CID: 194173),3′-Sialyllactose (PubChem CID: 123914),6′-Sialyllactose (PubChem CID: 643987),LS-tetrasaccharide b (PubChem CID: 53477864)

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