Characterization of an inactivated whole-virus bivalent vaccine that induces balanced protective immunity against coxsackievirus A6 and A10 in mice.

•A bivalent CVA6 and CVA10 vaccine induced systemic antibody titers comparable to those of monovalent vaccines.•The bivalent vaccine could induce strong antigen-specific polyfunctional T cell immune responses.•Passive transfer of antisera from vaccinated mice protected recipient mice against virus challenge.•Maternal antibody could afford protection against challenge with homologous or heterologous CVA6, CVA10, or CVA6/CVA10.•There was no immunological interference between the CVA6 and CVA10 components of the bivalent vaccine.