Repurposing of commercially available anti-coccidials identifies diclazuril and decoquinate as potential therapeutic candidates against Besnoitia besnoiti infection.

•A panel of commercially available drugs in cattle were tested against Besnoitia besnoiti in vitro.•Decoquinate and diclazuril showed the best results in terms of efficacy.•Decoquinate primarily affected the parasite mitochondrium.•Diclazuril interfered in cytokinesis of daughter zoites.•Decoquinate and diclazuril treatments do not act parasiticidal.